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sony id7000 spectral analyser  (Sony Biotechnology)


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    Structured Review

    Sony Biotechnology sony id7000 spectral analyser
    Sony Id7000 Spectral Analyser, supplied by Sony Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 743 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sony+id7000/ID7000+Spectral+Cell+Analyzer/pm42302662-597-5-5
    Average 99 stars, based on 743 article reviews
    sony id7000 spectral analyser - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Allogeneic CRISPR-Engineered CAR-T Cells Drive Potent Antitumor Activity in Solid Tumors
    Article Snippet: The bioluminescence signal flux was analyzed using the Living Image Software (Revvity) as photons per second per square centimeter per steradian (photons/s/cm 2 /sr). .. Flow cytometry was conducted using the Sony ID7000, and the data were analyzed with FlowJo software. .. For the mouse model studies, the Xenogen IVIS Lumina (PerkinElmer) was utilized, and the Living Image Software (Revvity) was employed to assess the bioluminescence signal flux.

    Article Title: Booster vaccination improves the durability of antibody-secreting plasma cells
    Article Snippet: For intracellular staining, cells were first stained with surface antibodies and then fixed with 4% formaldehyde (PFA, Thermo Scientific, Cat#: 28908) for 15 minutes at room temperature, washed once with PBS, then fixed with True-NuclearTM Transcription Factor Buffer Set (BioLegend, Cat# 424401) for 20 minutes at 4°C and kept in True-Nuclear 1X Perm Buffer for downstream intracellular staining. .. Flow cytometry data was acquired on BD FACSymphony A5, BD LSRFortessa X-20 using FACS-Diva software (BD Biosciences), or on Sony ID7000 using its system software. .. Sorting was performed using BD FACSAria Fusion Flow Cytometer and data acquired using FACS-Diva software (BD Biosciences).

    Article Title: Protocol for generating high-quality, 45-color spectral flow cytometry data for unsupervised clustering to investigate aging in human PBMCs
    Article Snippet: .. Using a Sony ID7000-optimized 45-color panel, we present a protocol for acquiring and preprocessing high-quality, high-parameter spectral flow cytometry data. ..

    Software:

    Article Title: Allogeneic CRISPR-Engineered CAR-T Cells Drive Potent Antitumor Activity in Solid Tumors
    Article Snippet: The bioluminescence signal flux was analyzed using the Living Image Software (Revvity) as photons per second per square centimeter per steradian (photons/s/cm 2 /sr). .. Flow cytometry was conducted using the Sony ID7000, and the data were analyzed with FlowJo software. .. For the mouse model studies, the Xenogen IVIS Lumina (PerkinElmer) was utilized, and the Living Image Software (Revvity) was employed to assess the bioluminescence signal flux.

    Article Title: Palmitic Acid Promotes Antiviral Innate Immunity via ZDHHC20-Mediated CMPK2 Palmitoylation.
    Article Snippet: .. Cells were detected using a SONY ID7000, and the data were analyzed using FlowJo software (Tree Star). .. Cells were harvested and lysed using cell lysis buffer (Cell Signaling Technology) supplemented with a protease inhibitor cocktail set (Millipore Sigma).

    Article Title: CD163 and Tim-4 identify resident intestinal macrophages that are spatially regulated by TGF-β.
    Article Snippet: For intracellular antibody staining, fixed cells were permeabilized with Permeabilization Buffer (Thermo Fisher Scientific) and stained with CD63 (NVG-2) and CD206 (C068C2) from BioLegend and pro-IL-1β (NJTEN3), TNF-α (MP6-XT22), and iNOS (CXNFT) from Thermo Fisher Scientific. .. Cell acquisition was performed on an LSRFortessa running FACSDIVA software (BD) or SONY ID7000 (SONY). ..

    Article Title: Polyethylene terephthalate nanoplastics promote gastric cancer progression by inhibiting the TNF-α/JAK1/STAT1 signaling pathway and shaping an immunosuppressive microenvironment.
    Article Snippet: Single cells were washed and stained with antibodies at room temperature for 30 min. Dead cells were excluded by staining with the Zombie N Fix & Viability Kit for 30 min (BioLegend, USA). .. Fluorescence data were acquired on a SONY ID7000 (Sony, Japan) and analyzed using FlowJo software. ..

    Article Title: Booster vaccination improves the durability of antibody-secreting plasma cells
    Article Snippet: For intracellular staining, cells were first stained with surface antibodies and then fixed with 4% formaldehyde (PFA, Thermo Scientific, Cat#: 28908) for 15 minutes at room temperature, washed once with PBS, then fixed with True-NuclearTM Transcription Factor Buffer Set (BioLegend, Cat# 424401) for 20 minutes at 4°C and kept in True-Nuclear 1X Perm Buffer for downstream intracellular staining. .. Flow cytometry data was acquired on BD FACSymphony A5, BD LSRFortessa X-20 using FACS-Diva software (BD Biosciences), or on Sony ID7000 using its system software. .. Sorting was performed using BD FACSAria Fusion Flow Cytometer and data acquired using FACS-Diva software (BD Biosciences).

    Article Title: CD163 and Tim-4 identify resident intestinal macrophages that are spatially regulated by TGF-β
    Article Snippet: For intracellular antibody staining, fixed cells were permeabilized with Permeabilization Buffer (Thermo Fisher Scientific) and stained with CD63 (NVG-2) and CD206 (C068C2) from BioLegend and pro-IL-1β (NJTEN3), TNF-α (MP6-XT22), and iNOS (CXNFT) from Thermo Fisher Scientific. .. Cell acquisition was performed on an LSRFortessa running FACSDIVA software (BD) or SONY ID7000 (SONY). ..

    Incubation:

    Article Title: Matrix Viscoelasticity Regulates Dendritic Cell Migration and Immune Priming.
    Article Snippet: For blocking, human c block (BioLegend, 422302) and Brilliant Stain Buffer (BD, 63794) were added to the cells and incubated at 4◦C for 10 min. ubsequently, the corresponding antibodies, diluted 1:100, were irectly added to the cell solution. .. After a 30-min incubation at ◦C, the cells were washed twice with FACS buffer, resuspended n FACS buffer, and analyzed using a Sony ID7000. ..

    FACS:

    Article Title: Matrix Viscoelasticity Regulates Dendritic Cell Migration and Immune Priming.
    Article Snippet: For blocking, human c block (BioLegend, 422302) and Brilliant Stain Buffer (BD, 63794) were added to the cells and incubated at 4◦C for 10 min. ubsequently, the corresponding antibodies, diluted 1:100, were irectly added to the cell solution. .. After a 30-min incubation at ◦C, the cells were washed twice with FACS buffer, resuspended n FACS buffer, and analyzed using a Sony ID7000. ..

    Article Title: Booster vaccination improves the durability of antibody-secreting plasma cells
    Article Snippet: For intracellular staining, cells were first stained with surface antibodies and then fixed with 4% formaldehyde (PFA, Thermo Scientific, Cat#: 28908) for 15 minutes at room temperature, washed once with PBS, then fixed with True-NuclearTM Transcription Factor Buffer Set (BioLegend, Cat# 424401) for 20 minutes at 4°C and kept in True-Nuclear 1X Perm Buffer for downstream intracellular staining. .. Flow cytometry data was acquired on BD FACSymphony A5, BD LSRFortessa X-20 using FACS-Diva software (BD Biosciences), or on Sony ID7000 using its system software. .. Sorting was performed using BD FACSAria Fusion Flow Cytometer and data acquired using FACS-Diva software (BD Biosciences).

    Fluorescence:

    Article Title: Polyethylene terephthalate nanoplastics promote gastric cancer progression by inhibiting the TNF-α/JAK1/STAT1 signaling pathway and shaping an immunosuppressive microenvironment.
    Article Snippet: Single cells were washed and stained with antibodies at room temperature for 30 min. Dead cells were excluded by staining with the Zombie N Fix & Viability Kit for 30 min (BioLegend, USA). .. Fluorescence data were acquired on a SONY ID7000 (Sony, Japan) and analyzed using FlowJo software. ..



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